Investigate Antibacterial Bioprocess Kinetics of Wood Acid against Pseudomonas Aeruginosa, Enterococcus Faecium, Clostridium Perfringens and Faecal Bacteria

Abstract

The cumulative number of varieties of infections, contaminations, and diseases caused by bacteria become a critical issue in the world. Besides, artificial chemical-based disinfectant also brings adverse effects to the environment, flora, and fauna. This research focuses to study pyroligneous acid as the alternative environmentally preferable disinfectant agent. A quantitative suspension test (EN 1040) was chosen to evaluate the antibacterial activity. On the obtaining results, the bioprocess kinetics of a variety of bacterial strains to pyroligneous acid-based disinfectants were further explored. This study resulted that the bioactivity reduction percentage and mean log reduction: Pseudomonas aeruginosa ATCC 9027 (100%, 6.0792), Enterococcus faecium ATCC 35667 (100%, 6.0000), Faecal bacteria (100%, 5.8921), and Clostridium Perfringens NCTC 8237 (100%, 5.9494) respectively. For bioprocess kinetics analysis, the specific reduction rate and halve rate:  Pseudomonas aeruginosa (2.7996, 4.0389), Enterococcus faecium (2.7631, 3.9863), Faecal bacteria (2.7134, 3.9146), and Clostridium Perfringens (2.7398, 3.9527) respectively. These findings showed that the pyroligneous acid-based disinfectant able to reduce the bacteria population significantly.